Glutamine Synthetase in Legumes: Recent Advances in Enzyme Structure and Functional Genomics

Biochemical and molecular analysis of glutamine synthetase gene in brassica napus L.

Recent Progress in Development of Tnt1 Functional Genomics Platform for Medicago truncatula and Lotus japonicus in Bulgaria

Legumes, as protein-rich crops, are widely used for human food, animal feed and vegetable oil production. Over the past decade, two legume species, Medicago truncatula and Lotus japonicus, have been adopted as model legumes for genomics and physiological studies. The tobacco transposable element, Tnt1, is a powerful tool for insertional mutagenesis and gene inactivation in plants. A large colle...

Recent Advances in High Cell Density Cultivation for Production of Recombinant Protein

This paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. High cell density cultures offer an efficient means for the economical production of recombinant proteins. However, there are still some challenges associated with high cell density cultivation (HCDC) techniques. A variety of strategies in several aspects including host design...

Enzymes with molecular tunnels.

As a result of recent advances in molecular cloning, protein expression, and X-ray crystallography, it has now become feasible to examine complicated protein structures at high resolution. For those enzymes with multiple catalytic sites, a common theme is beginning to emerge; the existence of molecular tunnels that connect one active site with another. The apparent mechanistic advantages render...

The regulation of glutamine synthesis in the food yeast Candida utilis: the purification and subunit structure of glutamine synthetase and aspects of enzyme deactivation.

Yeast glutamine synthetase was purified and shown to be an octameric globular protein (s = 15.4,fF0 = 1-29, mol. wt = 390000). It consists of two weakly-bound half molecules (s = 8.7, fFo = 1-35, mol. wt = 180500) and relatively harsh treatment is required to dissociate these octamers into component monomers (s = 3.8). Deactivation of the enzyme in vivo may include changes in the conformation o...